Tryptase activates PKB in inflammatory reaction in ECV304 cells

摘要

Tryptase is involved in proteinase-activated receptor-2 (PAR-2) mediated up-regulation of IL-8 expression. The present report showed the effects of tryptase on gene expression and activation, including up-regulation IL-8 expression. The expression of rnRNA for NF-kappa B first increased at I h after tryptase-treatment (1 ng/ml) and reached the plateau after 4 h. The NF-kappa B mRNA increased by 3-fold (n=3, P < 0.05), AP-1 by 2-fold (n=3, P < 0.05), and PKB by 10-fold (n=3, P < 0.05). However, tryptase-treatment did not affect the expression of JNK and p38 MAPK when compared with control cells at mRNA level. Furthermore, in addition to increasing phosphorylation of p38 MAPK, tryptase-treatment also increased phosphorylation of PKB by 2-fold at 15 min following the treatment. The up-regulation and phosphorylation of PKB by tryptase could be abolished by either phosphoinositol-3-kinase (PI3K) inhibitor (LY294002) at 10 mu M or antisense PKB cDNA transfection. The up-regulation of NF-kappa B expression could be inhibited by LY294002 and antisense PKB cDNA. These results indicate that tryptase can activate PI3K-PKB pathway and enhance IL-8 expression. (c) 2006 Elsevier B.V. All rights reserved.