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1BL/1RS translocation in durum wheat and its effect on end use quality traits

机译:1BL / 1RS在硬粒小麦易位及其对最终使用质量特征的影响

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摘要

The gluten proteins document the genotypic identity of a wheat variety, in addition to providing valuable clues about its ancestry and technological properties. In this study, an Indian durum wheat genotype B662 was identified to carry 1BL/1RS translocation and characterized further for its effect on end use quality traits. Comparison of the end use quality traits of B662 with five other durum cultivars without 1BL/1RS, showed decreased gluten content, lower swelling index of glutenins and low MSDS-SV indicating that, B662 with 1BL/1RS is not good for pasta making. In F-2:3 seeds from a durum wheat cross between the 1BL/1RS cultivar B662 and HI8498 without the translocation, the secalin Sec-1 loci segregated in theoretically expected 3:1 proportion and were inherited as a block of the rye chromosome arm. The analysis of F-2:3 harvests for the two most important durum wheat quality tests showed that the presence of 1BL/1RS translocation did not alter the grain protein content values, but was associated with significant reduction of micro SDS-sedimentation volume indicating inferior quality, thus limiting the commercial exploitation of durum wheat genotypes with 1BL/1RS translocation. The cautious use of rye translocation in Indian durum wheat breeding is suggested.
机译:面筋蛋白记录了小麦品种的基因型特征,此外还提供了有关其祖先和技术特性的宝贵线索。在本研究中,一个印度硬粒小麦基因型B662被鉴定携带1BL/1RS易位,并进一步研究了其对最终使用品质性状的影响。将B662与其他五个不含1BL/1RS的硬粒小麦品种的最终使用品质性状进行比较,结果表明,面筋含量降低,谷蛋白溶胀指数降低,MSDS-SV较低,表明含有1BL/1RS的B662不适合制作意大利面。在没有易位的1BL/1RS品种B662和HI8498之间的硬粒小麦杂交的F-2:3种子中,secalin Sec-1基因座按理论预期的3:1比例分离,并作为黑麦染色体臂的一个片段遗传。对两个最重要的硬粒小麦品质测试的F-2:3收获进行分析表明,1BL/1RS易位的存在不会改变籽粒蛋白质含量值,但与表明品质较差的微SDS沉降量显著降低有关,从而限制了1BL/1RS易位硬粒小麦基因型的商业开发。建议在印度硬粒小麦育种中谨慎使用黑麦易位。

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