Establishment and Gene Expression Characteristics of the Silk Gland Cell Line BmSG-SWU1 of Bombyx mori (Lepidoptera: Bombycidae)

摘要

A BmSG-SWU1 cell line was established from the silk gland tissues of newly hatched Bombyx mori L. larvae by performing primary cultures for 2 yr. The cell line was comprised of long and thin shuttle cells. The percentage of cells with the chromosome number 2n = 56 was 76.28%. Therefore, the cell line was considered a diploid cell line. Fingerprint analysis of BmSG-SWU1 cells and four other cell lines indicated that BmSG-SWU1 cells had a specific fingerprint, and that the genetic relationship between BmSG-SWU1 cells and the original silk gland tissue was the closest. We used whole genome microarray data, reverse transcription-polymerase chain reaction and fluorescence quantitative real-time-polymerase chain reaction to analyze the expression profile of BmSG-SWU1 cells. On day 3 of the fifth instar silk gland, the two known silk gland-specific genes Fib-L and P25 as well as 18 other highly expressed genes were found in the whole genome microarray data. Only some of these were expressed in the BmSG-SWU1 cell line, which showed that this cell line had maintained some gene expression characteristics of silk gland. The key cell cycle-control genes BmCyclinB and BmCyclinB3 of the G2 and M phase were expressed at low levels in silk glands. Contrarily, they were highly expressed in BmSG-SWU1cells, in the ovaries and other cell lines, which suggested that BmCyclinB and BmCyclinB3 are important for the transformation of BmSG-SWU1 cell line from endoreduplication to the mitotic cycle