Mechanism of Circular RNA RAD23B for Regulating Glycolysis and Proliferation of Ovarian Cancer Cells by Targeting MicroRNA-519b-3p

摘要

We investigated whether circRAD23B regulates glycolysis and proliferation of ovarian cancer cells by targeting miRNA-519b-3p. circRAD23B expression in ovarian cancer tissues was significantly higher than that in paracancerous tissues, and miRNA-519b-3p expression in ovarian cancer tissues was significantly lower than that in paracancerous tissues (P < 0.05). Transfection si-circRAD23B or miRNA-519b-3p mimics can greatly attenuate cell viability, glucose consumption, lactic acid level, and CyclinD1 protein level (P < 0.05), and increase p21 protein level (P < 0.05). Double luciferase reporter experiment confirmed that circRAD23B could function as a miRNA-519b-3p sponge molecule. Co-transfection of si-circRAD23B and anti-miRNA-519b-3p could greatly decrease cell viability, glucose consumption, lactic acid level, and CyclinD1 protein level (P < 0.05), and increase p21 protein level by comparison with si-circRAD23B + anti-miRNA-NC group (P < 0.05). Inhibiting circRAD23B expression can, in turn, inhibit glycolysis and the proliferation of ovarian cancer cells by up-regulating miRNA-519b-3p expression.