SYNTHESIS AND HYBRIDIZATION PROPERTIES OF OLIGONUCLEOTIDES INCLUDING 2 '-N-ALKOXYCARBONYL-2 '-AMINO-LNA DERIVATIVES

摘要

2'-Amino-LNA is an attractive material that helps in realizing the practical use of oligonucleotides because it can stabilize complexes with complementary strands and various substituents can be introduced on the 2'-amino group. Here, 2'-N-alkoxycarbonyl derivatives of 2'-amino-LNA were newly designed, synthesized, and incorporated into oligonucleotides. The results of UV-melting analysis indicated that 2'-N-alkoxycarbonyl-modified 2'-amino-LNA could enhance the stability of the duplex formed with single-stranded RNA and the triplex formed with double-stranded DNA.