The protective effect of procyanidinson tert-butyl hydroperoxide induced human hepatocyte HL7702 cell injury

摘要

The present study aims to investigate the mechanism of the protective action of Procyanidins (PC) against tertiary butyl hydroperoxide (TBHP)-induced cytotoxicity and cell death in human hepatocyte-derived cell line HL7702 cells. Incubation of HL7702 cells with PC prevented TBHP-induced loss in cell viability and reduction in GSH/GSSG ratio and activities of antioxidant enzymes have also been found to be prevented by PC. Western blot was used to evaluate the levels of cytochrome c, Bcl-2, Bax, Sirt3 and IDH2. The results showed that PC effectively protect TBHP-treated HL7702 cells from death, and increase superoxide dismutase activity and significantly decrease malondialdehyde production. PC increased the ratio of GSH/GSSG and NADPH/NADP(+), and reduced the release of cytochrome c from mitochondria and decreased the Bax expression, and also increased the expression of Bcl-2, IDH2 and Sirt3. PC prevented TBHP-induced oxidative stress injury in HL7702 cells and functioning via Sirt3-dependent mitochondria pathways.