Rapid purity check method for susceptibility testing of M. tuberculosis complex with the MGIT 960 system.

摘要

The Bactec MGIT 960 system is a rapid and reliable automated method for drug susceptibility testing of Mycobacterium tuberculosis complex (MTBC) that yields a high percentage of agreement with the standard method. The microscopic cord morphology of M. tuberculosis in liquid medium is characteristic, and readily differentiates MTBC from nontuberculous mycobacteria (NTM). The goals of this study were to describe the microscopic and macroscopic growth morphology of MTBC in antimicrobial-containing MGIT tubes and to evaluate the usefulness of the growth appearance during purity checking. The macroscopic cotton wool-like appearance of MTBC isolates in isoniazid (INH), streptomycin (SM), rifampin (RMP), and ethambutol (EMB)-containing tubes was observed in 97, 90, 93, and 71% of the isolates, respectively. The percentage of typical cord, loose, or frayed rope microscopic features in smears prepared from MTBC-positive cultures of INH, SM, RMP, and EMB-containing tubes was 96, 86, 97, and 71%, respectively. The sensitivity of the macroscopic morphology for predicting the purity of drug-containing MGIT tubes was 93%, while the microscopic morphology predicted the purity with a sensitivity rate of 92%. We found that simply examining the macroscopic morphology of the antimicrobial-containing MGIT tubes of drug-resistant MTBC isolates is useful in preventing false resistant results of susceptibility testing by the MGIT 960 system.