Comparison of (99m)Tc-labeled PR81 and its F(ab') fragments as radioimmunoscintigraphy agents for breast cancer imaging.

摘要

OBJECTIVE: We digested anti-MUC1 monoclonal antibody PR81 to produce F(ab') fragments. A comparison was performed between the two radiolabeled PR81 and F(ab') fragments for breast tumor imaging in a mouse model. METHODS: The optimum conditions for pepsin digestion of PR81 were investigated in terms of enzymes: antibody ratio, digestion time duration and preserved immunoreactivity of the produced fragments. The F(ab') fragments were labeled with Technetium-99m using HYNIC as a chelator and tricine as a co-ligand. The immunoreactivity of the complexes was assessed by radioimmunoassay using MCF7 cells. Biodistribution and imaging studies were performed in female BALB/c mice with breast tumor xenograft at 4, 8 and 24 h post-administration. The PR81 was labeled with technetium-99m in the same way for comparison. RESULTS: The optimum time duration for PR81 digestion was found to be 28 h at an enzyme:antibody weight ratio of 1:20 that resulted in 95.2 +/- 4.7% purity. The labeling of intact PR81 and its F(ab') fragments were 87.6 +/- 4.2 and 76.1 +/- 3.3% after 1 h, respectively (p value <0.05). The percentage of immunoreactivity of F(ab') fragments and intact PR81 were 75.4 +/- 2.1% and 85.7 +/- 2.9%, respectively (p value <0.05). The biodistribution and imaging studies demonstrated localization of the fragments at 4 h post-administration with high sensitivity and specificity. CONCLUSION: The results showed that F(ab') fragment of PR81 is more suitable than intact PR81 for safer and more rapid detection of human breast cancer.