Effects Of Different Components Of Serum After Burn On The L-Type Calcium Channel Of Cultured Myocardial Cells

摘要

Objective: The objective of the study was to observe the effects of different components of serum after burn on the L-type calcium channel of cultured myocardial cells. Method: Different components of serum were separated from the rat 6 h post-burn. The myocardial cells were separated from healthy rat and cultured in vitro, and the activity of the L-type channel was recorded by the patch-clamp technique. Results: After burn, the serum components enhanced the open action of the L-type calcium channel of cultured myocardial cells by increasing open-state probability, prolonging open time, shortening closed time, and enhancing current amplitude. Among the components, the low molecule (< 8 kD) fraction and lipid component were more effective. The effects of the lipid component could be suppressed by superoxide dismutase. Conclusion: After burn, some serum components could influence the activity of the L-type calcium channel, which might be an important cause of the dysfunction in cardiovascular system after burn.