Effect of ovarian steroids and oxytocin on the production of prostaglandin E2, prostaglandin F2alpha and endothelin-1 from cow oviductal epithelial cell monolayers in vitro.

摘要

Oviductal epithelial cells were collected from 8 non-pregnant Holstein cows during the follicular phase and cultured in M199 under standard culture conditions until monolayer formation. Cells in first passage were incubated for 24 or 48 h with 500 ng/ml progesterone, 1 ng/ml oestradiol-17beta, 10-9 M oxytocin or a combination of oestradiol and progesterone. Administration of oestradiol significantly increased the production of PGE2, PGF2alpha and endothelin-1. However, simultaneous administration of progesterone blocked the effect of oestradiol. Oxytocin did not have any effect on oviductal productions of PGs or endothelin-1. It is suggested that locally transferred oestradiol from the ovarian follicles may be important for oviductal contraction and gamete/zygote transport during the peri-ovulatory period.