Effective enhancement of resistance to Phytophthora infestans by overexpression of miR172a and b in Solanum lycopersicum

摘要

AbstractMain conclusionOverexpression of miR172a and b in tomato (Solanum lycopersicum) Zaofen No. 2 increased resistance toPhytophthora infestansinfection by suppressing of an AP2/ERF transcription factor.The miR172 family has been shown to participate in the growth phase transition, flowering time control, abiotic and biotic stresses by regulating the expression of a small group of AP2/ERF transcription factors. In this study, the precursors of miR172a and b were cloned from tomato,Solanum pimpinellifoliumL3708. We used the degradome sequencing to determine the cleavage site of miR172 to a member of the AP2/ERF transcription factor family (Solyc11g072600.1.1). qRT-PCR results showed that the expression ofAP2/ERFwas negatively correlated with the expression of miR172 inS. pimpinellifoliumL3708 infected withPhytophthora infestans.Overexpression of miR172a and b inS. lycopersicumZaofen No. 2 conferred greater resistance toP. infestansinfection, as evidenced by decreased disease index, lesion sizes, andP. infestansabundance. The SOD and POD play important roles in scavenging late massive ROS in plant–pathogen interaction. Malonaldehyde (MDA) is widely recognized as an indicator of lipid peroxidation. Membrane damage in plants can be estimated by measuring leakage of electrolytes, which is evaluated by determining relative electrolyte leakage (REL). Less H2O