Expression of Cloned dapB Gene of Pseudomonas aeruginosa in E. coli BL 21

摘要

DapB codes for enzyme dihydrodipiconilate reductase catalyze an essential step in synthesis of diaminopimelic acid (DAP)-an essential component in the cell wall of many bacteria including P. aeruginosa. In its absence, microbes get lysed eventually. Its absence in human system makes it an attractive target for targeting microbial pathogen. Owing to this, expression of coined dapB of Pseudomonas aeruginosa was done in E. coli BL 21. ^