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Birth weight and birth rate of heavy calves conceived by transfer of invitro or in vivo produced bovine embryos

机译:通过体外或体内产生的牛胚胎的转移设想的重犊牛的出生体重和出生率

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The aim of this study was to evaluate the difference in birth weight and gestation length between Japanese Black calves obtained from transfer of bovine embryos produced in vitro (IVP) and those developed in vivO (IVD). An additional objective was to clarify the sire effect on birth weight and gestation length and to examine the birth rate of heavier calves, Two Japanese Black bulls breed at our experimental station were used as a semen source for production of NP and ND embryos. Thirty-eight Japanese Black heifers and cows of various genetic backgrounds were used as embryo donors for IVD embryos. Ovaries for IVP embryos were collected at random at a local slaughterhouse from Japanese Black cattle of various genetic backgrounds. IVP embryos were produced using co-culturing with cumulus cells in 5% CS + TCM 199. Both the IVD and NP embryos were transferred non-surgically to Holstein recipients on day 7 +/- 1 of estrous cycle. In this study, the birth weights and gestation lengths of half-sib single calves for bull A and B were analyzed.
机译:这项研究的目的是评估通过移植体外产生的牛胚胎(IVP)和体内存活的牛胚胎(IVD)获得的日本黑小牛的出生体重和妊娠长度的差异。另一个目的是弄清父亲对出生体重和妊娠长度的影响,并检查较重的犊牛的出生率。在我们的实验站,将两只日本黑公牛作为精子来生产NP和ND胚胎。 38种具有不同遗传背景的日本小母牛和母牛被用作IVD胚胎的胚胎供体。从当地的各种遗传背景的日本黑牛的屠宰场中随机收集了IVP胚胎的卵巢。通过在5%CS + TCM 199中与卵丘细胞共同培养来生产IVP胚胎。在动情周期的第7 +/- 1天,将IVD和NP胚胎均通过非手术方式转移到Holstein受体。在这项研究中,分析了公牛A和牛B的半同胞单犊牛的出生体重和妊娠长度。

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