A novel micro pressurized liquid extraction method for very rapid solid sample preparation

摘要

A novel micro pressurized liquid extraction (mu PLE) method is introduced, which employs rapid heating in a static mode to remove analytes from 5-10 mg samples in as little as 10 seconds using only 125 mu L of solvent. For example, using pharmaceutical tablets and green tea leaves as test samples, 99 +/- 5% of acetylsalicylic acid and 97 +/- 4% of acetaminophen (both n = 4) were each extracted into methanol from tablets in 10 seconds at 150 degrees C. For green tea, 100 +/- 20% (n = 4) of the caffeine present could be extracted into methanol after only 20 seconds at 275 degrees C. These extraction results compared very well with those of conventional PLE trials on the same samples, although mPLE was much faster and less solvent intensive. As an application, mPLE was found to extract 101 +/- 7% (n = 6) of Fluoxetine Hydrochloride from dried blood spot analysis cards after only 20 seconds at 250 degrees C. The instrumentation and procedure required for this technique are relatively simple, inexpensive, and can easily be adapted in most laboratories. Overall, the results indicate that mPLE may provide a useful method for rapidly preparing solid samples for analysis using little solvent. The potential advantages and limitations of the method are presented and discussed.