Expedient preparative isolation, quantification and characterization of limonoids from Neem fruits

摘要

Major basic limonoids from Neem fruits such as azadirone, epox-yazadiradione and azadiradione were isolated in preparative scale using an automated and rapid medium pressure liquid chromatography (MPLC)-based protocol. They were individually quantified using HPLC. An LC-ESI-MS/MS-based rapid identification technique was developed for the high-throughput screening of these limonoids in phytochemical extracts.