FROZEN STORAGE OF RAM SEMEN .1. PROCESSING, FREEZING, THAWING AND FERTILITY AFTER CERVICAL INSEMINATION [Review]

摘要

Research up to 1993 is reviewed. Various methods for processing, freezing and thawing of semen have been elaborated and their effect on survival and fertility of spermatozoa have been examined in vitro or in fertility tests. Diluents initially used for freezing bull semen had their limitations and were modified or replaced by new media, The freezing diluents investigated included citrate-sugar-, milk-, lactose-, saccharose-, raffinose-, tris-based and other media containing protective agents (mainly glycerol and egg yolk), and other substances including antioxidants. Satisfactory sperm survival rates were obtained with a number of diluents and different combinations of the factors studied.