A facile transport assay for H~+ coupled membrane tramsport using fluorescence probes

摘要

A facile activity assay for an H~+-coupled transporter using flores-cent probes was developed with an H~+-coHpled manganese transporter (MntH) as a model. Making use of coupled-proton transport, the transport activity (H~+/Mn~(2+) cotransport) can be directly determined via fluorescence intensity changes of the probe, 5-(ani-6)-carboxyfluorescein (5(6)-FAM). The approach of using highly sensitive inorescence probes provide a more simple and convenient assay method for the determination of proton-coupled metal-ion uptake by transporters.