A comprehensive review on genetically modified fish: key techniques, applications and future prospects

摘要

Genetically modified fish is a general term used for whole fish whose DNA has been artificially altered by directly integrating (or deleting) single or multiple genes to introduce or modify a targeted character. Different techniques have been performed to produce genetically modified fish. Importantly, the introduction of various types of fluorescent proteins has dramatically expanded the range of applications of genetically modified fish. Currently, genetically modified fish have been widely employed as experimental models for medical science, pharmacology and environmental toxicology. The commercial application of genetically modified fish in aquaculture is still up for debate, although the first commercialization of genetically modified fish, GloFish, was in 2003, and further, the United States and Canada approved the commercial production and sale of the AquAdvantage Salmon. In this review, we discuss the principles and developmental history of fish transgenesis via gene transfer and gene-editing techniques, and their applications in diverse fields. Further, challenges and prospects associated with the genetically modified fish are discussed.