Detection of Mec cassette gene among coagulase negative Staphylococci isolated from different Clinical Cases

摘要

Coagulase Negative Staphylococci (CONS) are considered as a major pathogen of nosocamial infections. The aim of this study was to identify the types of staphylococcus cassette chromosome Mec (Sccmec) among clinical methicillin-Resistance CONS isolates collected from different clinical cases from AL Diwanyhia teaching hospital through February to June 2018. Antimicrobial susceptibility tests of 5. lentus and S. xylosus isolates were performed by the disc diffusion method. Twenty isolates were screened for methicillin resistance based on resistance to cefoxitin (30ug) disc and presence of mec gene. Determination of Sccmec typing by PCR method 20 isolates were resistance to methicillin according to phenotypic and Genotype that presence of mec A gene. PCR analysis showed that 12 isolates carried Sccmec type II, 12 isolates were type III, 11 isolates type IV, 13 isolates type V, all isolates could not be type I. Increased prevalence of Sccmec types in community are causes of an increase in antibiotic resistance among microorganism.