Effects of 5-Aza-2'-deoxycytidine on hormone secretion and epigenetic regulation in sika deer ovarian granulosa cells

摘要

5-Aza-2'-deoxycytidine (5-Aza-dC), an inhibitor of DNA methyltransferases, is an effective treatment for various cancers and has improved the development rate of cloned embryos. Previous studies have reported the effect of 5-Aza-dC on fibroblasts; however, the mechanism whereby 5-Aza-dC affects sika deer granulosa cells and hormone secretion is presently unknown. Here, we showed that the cell cycle after treatment with different doses of 5-Aza-dC was significantly altered. The number of cells in the S phase was significantly increased in response to a concentration of 0.1 mu M 5-Aza-dC. The rate of apoptosis was increased when cells were treated with 0.1 mu M and 5 mu M 5-Aza-dC. We showed that the protein level of H3K9me2 was significantly decreased in response to 5-Aza-dC. The activity levels of DNA methyltransferase were reduced by a moderate dose of 5-Aza-dC. Furthermore, the secretion of E-2 and P-4 was influenced by different doses of 5-Aza-dC. Our study suggested that 5-Aza-dC affected hormone secretion in sika deer granulosa cells through cell development and epigenetic regulation. The findings of this study lay the foundation for further epigenetic studies in sika deer.