Development of a new TaqMan-based PCR assay for the specific detection and quantification of Simkania negevensis

摘要

Simkania negevensis is an emerging Chlamydia-like bacterium related to human respiratory diseases. An early and accurate detection of this pathogen could be useful to monitor the potential infectious risks and to set suitable outbreak control measures. In Tunisia, distribution and abundance of S. negevensis remain until now largely unknown. In the present work, a qPCR assay, targeting the 168 rRNA gene, for fast detection and quantification of S. negevensis was developed and validated.