Facile synthesis of mesoporous copper silicate aggregates for highly selective enrichment of hemoglobin

摘要

The purification of histidine-rich proteins in complex biological samples has been an important topic for proteomics research and protein drug development. In order to achieve high selectivity and binding capacity for histidine-rich protein, novel mesoporous material of copper silicate have been synthesized via in-situ growth method with dendritic mesoporous silica as the template in a hydrothermal treatment process. The resulted materials maintain large pore size with ample adsorption sites. The maximum adsorption capacity for bovine hemoglobin is 4150 mg g-1. In addition, the formed mesoporous copper silicate aggregates showed excellent performance for the removal of highly abundant hemoglobin in human blood, permitting their potential applications in the pretreatment of complex biological samples.