FAME and RAPD analysis of selected Vicia taxa from eastern Anatolia, Turkey.

摘要

Fatty acid (FAs) and RAPD profiles were used to examine the phenotypic and genetic relationships among 12 Vicia taxa, namely V. villosa subsp. villosa, V. sativa subsp. nigra, V. faba subsp. minor var. minor, V. pannonica var. pannonica, V. cracca subsp. cracca, V. canescens subsp. variegata, V. cracca subsp. stenophylla, V. noeana var. noeana, V. hirsuta, V. sativa var. cordata, V. canescens subsp. gregaria, and V. sativa subsp. sativa. All the Vicia taxa were separated based on the presence and composition of 77 different FAs. Eight of the ten decamer primers examined were selected to explore the genetic variation. A total of 156 amplicons in the size range of 125-2500 bp were produced by eight different primers from the 12 Vicia taxa. Even though all Vicia species were differentiated by RAPD profiles, three genetically distinct groups were found among the species tested. This is the first study showing that RAPD and FAME analyses are useful methods for differentiation and classification of Vicia and perhaps other plant species and/or taxa as well..