Synergic combination of paclitaxel and irinotecan loaded poly(lactic-co-glycolic acid) nanocomplexes for MRI and the treatment of endometrial cancer

摘要

We have established dual paclitaxel (PTX)/superparamagnetic iron oxide (SPIO)-loaded PLGA-based nanoparticles for theranostic purposes. The fabricated nanoparticles exhibited a spherical morphology with a size of similar to 240 nm. The PTX and iron loading were 1.84 +/- 0.4 and 10.4 +/- 1.93 mg/100 mg, respectively. The relaxometry studies and phantom magnetic resonance imaging demonstrated their efficacy as T-2 contrast agents. A significant cellular uptake of nanoparticles by the endometrial Ishikawa cells was demonstrated using confocal laser scanning microscopy. While SPIO did not show any toxicity in endometrial Ishikawa cells, PTX-loaded nanoparticles indicated cytotoxic activity. Together, these advantages of multifunctional nanoparticles may be considered as future nanomedicines for simultaneous molecular imaging, drug delivery, and real-time monitoring of therapeutic response.