Analysis of the effectiveness of reused primary and secondary antibodies in Western blotting analysis

摘要

Western blot analysis is a common technique for detecting a target protein on a nitrocellulose membrane. The target protein is detected using specific antibodies. The positive signal can be visualized either by chemiluminescence or by a colorimetric reaction. Some specific antibodies can be produced in-house in high amounts and can be used without considering costs. However many antibodies (especially antibodies for detection tag fused proteins) must be purchased from a company. Unfortunately most of them are very expensive. Here we report the effectiveness of different antibodies after reusing them several times and also compare the influence of different storage conditions.