Recombinant human irisin regulated collagen II, matrix metalloproteinase-13 and the Wnt/beta-catenin and NF-kappa B signaling pathways in interleukin-1 beta-induced human SW1353 cells

摘要

Osteoarthritis (OA) is a degenerative joint disease that seriously affects the quality of life of patients. Irisin has been reported to regulate bone metabolism via the cellular autocrine mechanism and play a protective role in rat OA. In the present study, a SW1353 chondrosarcoma cell line was treated with interleukin (IL)-1 beta and irisin. The present study evaluated cell viability, expression levels of collagen II (Col II) and matrix metalloproteinase-13 (MMP-13), and activity of the Wnt/beta-catenin and NF-kappa B signaling pathways in treated SW1353 cells. The present results suggested that IL-1 beta could decrease Col II expression and increase MMP-13 expression at both the mRNA and protein levels, and also activate the Wnt/beta-catenin and NF-kappa B signaling pathways in SW1353 cells. By contrast, irisin was identified to reverse the effects of IL-1 beta in IL-1 beta-induced SW1353 cells. The present results suggested that irisin treatment may have a cartilage-protective role in an IL-1 beta-induced SW1353 cell model.