An improved micropropagation via nodal segments of Zingiber officinale

摘要

An improved micropropagation via nodal segments of Zingiber officinale was described in the present study. Nodal segments containing 1-3 nodes were obtained from 5-weeks old stock shoots and cultured on Murashige and Skoog medium (MS) (Murashige and Skoog, 1962) supplemented with 1 mg L-1 thidiazuron (TDZ) and 0.5 mg L-1 naphthaleneacetic acid (NAA), to promote shoot elongation from axillary buds. With this protocol, more than 90% of nodal segments containing one and two nodes formed axillary shoots, with 2.2-2.3 shoots per explant produced after 2 weeks of culture. When the shoots were proliferated on MS supplemented with 3 mg L-1 6-benzylaminopurine (BAP) and 0.2 mg L-1 NAA, 6.3 shoots per explant with well-developed roots, each shoot being 6.2 cm in length, were obtained after 5 weeks of proliferation. The plants were established in soil following acclimatization. Analysis of inter simple sequence repeat (ISSR) and random amplified polymorphic DNA (RAPD) did not detect any polymorphic bands in micropropagated plants. To the best of our knowledge, this is the first report on micropropagation using nodal segments of ginger native to China. Results reported here provide a technical support for micropropagation of Zingiber spp.