Mutualistic interaction between dichloromethane‐ and chloromethane‐degrading bacteria in an anaerobic mixed culture

Chen Gao; Kleindienst Sara; Griffiths Daniel R.; Mack E. Erin; Seger Edward S.; L?ffler Frank E.

摘要

Summary > The microbial mixed culture RM grows with dichloromethane (DCM) as the sole energy source generating acetate, methane, chloride and biomass as products. Chloromethane (CM) was not an intermediate during DCM utilization consistent with the observation that CM could not replace DCM as a growth substrate. Interestingly, cultures that received DCM and CM together degraded both compounds concomitantly. Transient hydrogen (H <sub>2</sub> ) formation reaching a maximum concentration of 205?±?13 ppmv was observed in cultures growing with DCM, and the addition of exogenous H <sub>2</sub> at concentrations exceeding 3000 ppmv impeded DCM degradation. In contrast, CM degradation in culture RM had a strict requirement for H <sub>2</sub> . Following five consecutive transfers on CM and H <sub>2</sub> , Acetobacterium 16S rRNA gene sequences dominated the culture and the DCM‐degrader Candidatus Dichloromethanomonas elyunquensis was eliminated, consistent with the observation that the culture lost the ability to degrade DCM. These findings demonstrate that culture RM harbours different populations responsible for anaerobic DCM and CM metabolism, and further imply that the DCM and CM degradation pathways are mechanistically distinct. H <sub>2</sub> generated during DCM degradation is consumed by the hydrogenotrophic CM degrader, or may fuel other hydrogenotrophic processes, including organohalide respiration, methanogenesis and H <sub>2</sub> /CO <sub>2</sub> reductive acetogenesis. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span>None </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" > 著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-22868/'>《Environmental microbiology 》</a> <b style="margin: 0 2px;">|</b><span>2017年第11期</span><b style="margin: 0 2px;">|</b> <span>共13页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Chen Gao&option=202" target="_blank" rel="nofollow">Chen Gao;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Kleindienst Sara&option=202" target="_blank" rel="nofollow">Kleindienst Sara;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Griffiths Daniel R.&option=202" target="_blank" rel="nofollow">Griffiths Daniel R.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Mack E. Erin&option=202" target="_blank" rel="nofollow">Mack E. Erin;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Seger Edward S.&option=202" target="_blank" rel="nofollow">Seger Edward S.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=L?ffler Frank E.&option=202" target="_blank" rel="nofollow">L?ffler Frank E.;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Center for Environmental BiotechnologyUniversity of TennesseeKnoxville TN 37996 USA;</p> <p>Center for Environmental BiotechnologyUniversity of TennesseeKnoxville TN 37996 USA;</p> <p>ParsonsDenver CO 80203 USA;</p> <p>Corporate Remediation GroupE. I. DuPont de Nemours and CompanyWilmington DE 19805 USA;</p> <p>The Chemours CompanyWilmington DE 19899 USA;</p> <p>Center for Environmental BiotechnologyUniversity of TennesseeKnoxville TN 37996 USA;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li> <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span> <a href="https://www.zhangqiaokeyan.com/clc/183.html" title="微生物学">微生物学 ;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> </p> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li 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MUHAMMAD ADNAN RASHID</a> <span> . 2018</span> </span> </div> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/patent-detail/06130499508692.html">LONG-TERM STORAGE MEDIUM FOR CULTURING OBLIGATE ANAEROBIC BACTERIA OR MICROAEROBIC BACTERIA UNDER AEROBIC ENVIRONMENT, AND METHOD OF DETECTING OBLIGATE ANAEROBIC BACTERIA OR MICROAEROBIC BACTERIA USING SAID MEDIUM</a> <b>[P]</b> . <span> 外国专利：  EP3786283A1 </span> <span> . 2021-03-03</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：用于在有氧环境下培养厌氧细菌或微生物细菌的长期储存介质，以及使用所述介质检测厌氧细菌或微生物细菌的方法 </span> </p> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/patent-detail/06130410081591.html">LONG-TERM STORAGE MEDIUM FOR CULTURING OBLIGATE ANAEROBIC BACTERIA OR MICROAEROBIC BACTERIA UNDER AEROBIC ENVIRONMENT, AND METHOD OF DETECTING OBLIGATE ANAEROBIC BACTERIA OR 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