Comparative Evaluation of Developed Carrier Based Bioformulations Bearing Multifarious PGP Properties and Their Effect on Shelf Life under Different Storage Conditions

摘要

The current study was undertaken to determine the bio-formulations of two economically viable carriers (talc and sugarcane bagasse) based preparations by using plant growth promoting (PGP) strains. A total of 20 bacterial isolates were recovered fromrice agricultural fields of Alibag and Chiplun, Maharashtra, India. Recovered bacterial isolates were screened on the basis of PGP properties like phosphate, zinc solubilization, siderophore, indole acetic acid and ammonia production. On the basis of plant growth promoting potential, two bacterial isolates i.e. A2 and CP 19 were selected and showed 98% homology with Providencia vermicola and Klebsiella pneumoniae based on 16S rDNA sequence analysis. The selected PGP strains were co-inoculated in combination with each other after finding the compatibility of both strains and designated as consortium Cl. The developed consortium was utilized for bio-formulation preparation with talc and sugarcane bagasse carrier, respectively. The viable cell count of both bio-formulations was determined at the end of 2nd, 4th, 11th, 18lh, 25th, 40th, 55th and 70th day of incubation at different storage conditions (at 4°C, room temperature and outdoor conditions). The comparative assessment of shelf life revealed thatthe viability of bacterial consortia Cl was found maximum in talc based bioformulations as compared to bagasse bioformulation after 70 days of storage (DAS). The consortia based talc bioformulation was found to confer a significant increase in the shelflife parameters under different conditions. The results of the present study suggest that talc based bioformulation of bacterial consortia of Providencia vermicola A2 and Klebsiella pneumoniae CP 19 can be used as an effective carrier for easy application, storage and can be applied at the site of action for economizing production process and longer shelf life.