Selective determination of cysteine by resonance light scattering technique based on self-assembly of gold nanoparticles

摘要

The interaction between cysteine and gold nanoparticles was studied. Through the covalent combination with the -SH group and the electrostatic binding with the -NH3+ group of cysteine, gold nanoparticles call self-assemble to form a network Structure, Which results in greatly enhanced resonance light scattering (RLS). The experimental results demonstrate that the RLS technique offers a sensitive tool for investigations of self-assembly of nanoparticles. On the other hand, the RLS method can be applied to selectively determine cysteine with high sensitivity and simple operation. The linear range of determination of cysteine is from 0.01 to 0.25 mu g/mL with the detection limit of 2.0 ng/mL (16.5 nM, 3 sigma). None of the amino acids found in proteins inteferes with the determination. (c) 2006 Elsevier Inc. All rights reserved.