Purification, Characterization, Antioxidant and Antitumour Activities of Polysaccharides from Apple Peel Pomace Obtained by Pre-pressing Separation

摘要

Purification, characterization, in vitro antioxidant and antitumour activities of polysaccharides from apple peel pomace obtained by pre-pressing separation were investigated. Apple peel polysaccharides (APP) were obtained by water extraction and ethanol precipitation. They were further purified by chromatography of Cellulose DEAE-52 and Sephadex G-150 to obtain three fractions (APP-1, APP-2 and APP-3). Both GC-MS and HPLC analysis showed that galactose and arabinose were two main components in the three fractions with an average molecular weight of 197 kDa, 395 kDa and 303 kDa, respectively. Uronic acids were only presented in APP-2 and APP-3, which were further confirmed by Fourier transform infrared spectroscopy. Besides, the results showed that the antioxidant activity measured by ferric-reducing power and scavenging of O_2~(·-), DPPH~· and OH~· and the antitumour activity examined by HepG2 cells were connected with the content of uronic acids. These findings provide scientific basis for comprehensive utilization of apple peel pomace.