GENETIC DIVERSITY IN SINGLE AND DOUBLE TYPE OF TUBEROSE (POLIANTHES TUBEROSA L.) GERMPLASM USING RANDOMLY AMPLIFIED POLYMORPHIC DNA (RAPD) MARKERS

摘要

Genetic variation among 21 tuberose genotypes were evaluated using seven randomly amplified polymorphic DNA markers. The results revealed that the RAPD markers produced much better reproducible bands and were more efficient in grouping germplasm. Polymorphic information content varied from 0.37-0.98 with an average value of 0.813, resolving power vary from 0.28 to 5.61 with an average 2.67, while the marker Index varied from 0.74 to 5.98 with an average value of 2.70, respectively. The number of RAPDfragments generated per primer set ranged from 1 to 7 with an average 3.57 bands per primer and fragment sizes varying from 180-1400 bp. A total of 19 polymorphic bands generated 100% polymorphism. All germplasms were clearly differentiated by their RAPD fingerprints. The Jaccard similarity indices (J) based on RAPD profiles were subjected to UPGMA cluster analysis. The dendogram generated by RAPD markers revealed four major groups and noted considerable amount of genetic variation among the genotypes.Genotypes namely Mexican White Double and Swarna Rekha cultivar were found to be more diverse in molecular analysis.