Synthetic Peptides-Based Indirect ELISA for the Diagnosis of Bovine Anaplasmosis

摘要

This paper presents a first initial approach to diagnose bovine anaplasmosis using a synthetic peptides based indirect ELISA. The capacity of the positivity values obtained for a peptide mixture is higher than the capacity of the same peptides used individually, in other words, the combination of both peptides increases their antigenic potential. In some cases, the positivity values of sera show an almost six-fold increase when the peptide mixture is used as an antigen compared to the crude antigen of the in-house indirect ELISA (iELISA). The sensitivity of the assay using the peptide mixture is 100%, while the confidence for specificity is 95%. The design and use of synthetic peptides for anaplasmosis might be implemented as an alternative to the crude extract or recombinant proteins, which may result more expensive and experimentally complicated.