Production of Milk Clotting Enzyme from Distiller's Sludge by Penicilliim camemberti using Response Surface Methodology

摘要

In this study, the production of milk clotting enzyme was carried out by the Distillers yeast sludge using the fungal culture Penicillium camemberti. Effects of different substrates on the production of milk clotting enzyme were studied under stationary and shaking conditions. Highest milk clotting activity was observed in the medium containing casein under static conditions. Central composite design experiments werecarried out to optimize the parameters that brings outhigh yield milk clotting enzyme. Maximum milk clotting activity of 0.785units/mg was obtained at optimum process conditions namely initial substrate concentration 15 g/l, initial pH 5.9, temperature 39°C and biomass concentration 15g/l. The Logistic model for cell growth and Leudeking-Piret model for product formation kinetics were evaluated for the prediction of experimental data.