Amperometric biosensor sensitive to glucose and lactose based on co-immobilization of ferrocene, glucose oxidase, beta-galactosidase and mutarotase in beta-cyclodextrin polymer

摘要

An amperometric biosensor sensitive to glucose and lactose has been developed by immobilizing glucose oxidase (GOD), beta-galactosidase, mutarotase and ferrocene in beta-cyclodextrin polymer. The ferrocene is included in the cavities of the beta-cyclodextrin polymer through a host-guest chemical reaction whereas glucose oxidase, beta-galactosidase and mutarotase are cross-linked with the beta-cyclodextrin polymer. Cyclic voltammetry and amperometric measurement have been employed for the first time to show the efficacy of electron transfer between immobilized glucose oxidase and a glassy carbon electrode via ferrocene included in the cavities of beta-cyclodextrin polymer. Performance and characteristics of the biosensor were evaluated with respect to response time, detection limit, selectivity, and dependence on applied potential, temperature and pH as well as operating and storage stability. The stability of the enzyme membrane was greatly enhanced by cross-linking of the enzymes with beta-cyclodextrin polymer because of the water absorbability of the beta-cyclodextrin polymer. (C) 1998 Elsevier Science B.V. [References: 25]