An acoustic based approach to evaluate the effect of different diet lengths on feeding behavior of Litopenaeus vannamei

摘要

Studying shrimp feeding behavior is challenging and, traditionally, studies have been using direct visual observation or video recordings with image analysis under laboratory conditions. Passive acoustics monitoring (PAM) is a non-invasive approach to study feeding behavior of penaeids, as their mandibles emit clicking sounds during feed ingestion. Considering the wide variation available in shrimp diet lengths, our knowledge is still limited about their effects on feeding behavior. The present study was designed to use acoustics to evaluate the feeding behavior of Litopenaeus vannamei fed different diet lengths. A commercial pelleted diet with similar diameter (2.4 mm) and three different lengths was used as treatments "small" (2.35 mm), "medium" (4.26 mm) and "large" (8.42 mm). Three separate trials were performed with fastened shrimp (20 g), using omnidirectional hydrophones and external audio recorders. Our approach started with the characterization of the click acoustic parameters produced by three shrimps when fed the different diet lengths in anechoic chambers (15 L). We then analyze the number of clicks produced per pellet and duration of the clicking activity when shrimp fed a single pellet in individual tanks (1 L). Finally, the acoustic energy of feeding activity and feed consumption, as well as theirs relationship to predict feed intake was evaluated in aquaria (70 L) with five shrimps. The acoustic parameters of isolated clicks were not affected by the different diet lengths, however, doubling the length of the pellet resulted in doubling the number of clicks emitted during feeding activity. The duration of clicking activity per pellet indicated that small pellets are consumed 2.9 and 4.7 times faster than medium and large pellets, respectively. Furthermore, feeding acoustic energy of small pellets decreased rapidly after feed offer, as their faster consumption probably contributed to shrimps achieved satiety earlier than larger diets. Feed consumption was similar among diets length, but significantly correlated with the feeding acoustic energy emitted by L. vannamei, which is a breakthrough in feeding behavioral research. The results highlight the potential and possibilities of an acoustic approach to estimate feed consumption, as well as an alternative to traditional methods in feeding behavioral studies to improve efficiency of feed management.