The maintenance of a PARAFAC calibration and the second-order property: application to the determination of ciprofloxacin in presence of enrofloxacin by excitation-emission fluorescence

摘要

Calibration maintenance from day to day is an interesting problem when an analytical method is proposed for routine daily use. In this work the performance criteria of the fluorescence in the determination of quinolones by means of the parallel factor analysis (PARAFAC) model has been established according to the ISO standards and the 2002/657/EC European Decision. The presence of these substances in animals for human consumption is regulated and forbidden in poultry producing eggs for human consumption (Regulation No. 1181/2002).The paper shows that the capability of detection of the fluorescence technique is satisfactory with respect to the legal regulation. The detection limits found for ciprofloxacin without and with interferent were 33 and 27 mu g l(-1) when the risks of false positive and false negative have been fixed at 5%. The corresponding hypothesis test has shown that there is neither a constant error nor a proportional error in the models constructed with PARAFAC, that is, the method can be considered accurate. (c) 2004 Elsevier B.V. All rights reserved.