Simultaneous determination of p-agonists and monitoring in bovine tissues by liquid chromatography-tandem mass spectrometry

摘要

The misuse of P-agonists leads to a potential risk to public health and is forbidden in many countries. We developed a rapid, sensitive and reliable multi-residue detection method for zilpaterol, ractopamine, and clenbuterol in bovine tissues by liquid chromatography-tandem mass spectrometry. Residues were extracted in ethyl acetate after protein precipitation, and then analyzed by the developed method. Good linearities (R~2 > 0.99) were observed, and the recoveries of zilpaterol, ractopamine, and clenbuterol were 99%, 74%, and 102%, respectively. The limits of quantitation for zilpaterol. ractopamine, and clenbuterol were 1.3, 5.0, and 1.7 ng/g. respectively. The method is also applied successfully to bovine tissues within the Korean National Residue Programme. None of the 3 P-agonists were detected from 50 domestic samples. However, zilpaterol (6.3 ng/g) was quantified in one out of the 50 imported samples. The application of this method will be helpful in quality control analy sis of P-agonists residues in bovine tissues.