Significant Changes in Morpho-physiological and Haematological Parameters of Carassius gibelio (Bloch, 1782) (Actinopterygii: Cyprinidae) as Response to Sporadic Effusions of Industrial Wastewater into the Sazliyka River, Southern Bulgaria

摘要

Basic morphological, physiological and haematological parameters adult, sexually mature individuals of Carrasius gibelio were studied in three water basins in Southern Bulgaria. Two of them are situated in the region of the town of Galabovo: the Sazliyka River, near the sluice gates, where water is diverted to TPS Brikel (site A) and the Rozov Kladenets Reservoir (site B). The third site, the Vacha River (site C), is less disrupted (conventional control). In C. gibelio specimens inhabiting site A the lowest values of standard length (18.04 cm), body weight (121.27 g) and condition factor (2.06) and high values of spleen-somatic index (0.37) and ren-somatic index (0.59) were measured. All of the haematological parameters, except PCV (0.27 l/L) and MCH (83.19 pg), in individuals inhabiting site A (RBC - 1.327 х 10~(12)/L, WBC - 2923 N/mm~3, Hb - 10.91 g/dL, MCHC - 408.85 g/L and MCV - 208.62 fL) were significantly different when compared to the specimens from the other two sites: site B (RBC - 1.113 х 10~(12)/L, WBC - 2100 N/mm~3, Hb - 9.01 g/dL, MCHC - 353.58 g/L and MCV - 239.62 fL) and site C (RBC - 1.116 х 10~(12)/L, WBC - 2183 N/mm~3, Hb - 9.32 g/dL, MCHC - 359.21 g/L and MCV - 249.31 fL). The changes in the differential blood count of specimens of C. gibelio from site A were characterised by neutrophilia (28.91%), eosinophilia (0.91%), monocytosis (5.41%) and lymphocytopenia (64.77%). These contrasted to the results observed for the other two populations, inhabiting sites B and C: neutrophils - 6.07% / 5.87%, eosinophils - 0.67% / 0.63%, monocytes - 2.45% / 2.77% and lymphocytes - 90.81% / 90.73%, respectively. This changes indicated intensive stress, caused by the industrial pollutants released (sporadic effusions) in the Sazliyka River from TPS Brikel.