Determining morphine in biologic fluids of rats by gas chromatography-mass spectrometry

摘要

This study describes a sensitivity and selectivity approach based on gas chromatography-mass spectrometry to determinate morphine levels in rat biologic fluids.Optimized experimental conditions of solid-phase extraction (SPE),including pH of the sample,solvent composition,and various sorbents,are examined herein.The largest recovery is greater than 94.0% for morphine,is achieved with a mixed sorbent C_8/SCX and mixed elution CHCl_3/iso-propanol (9:1) at pH 6.8.Acetylation accomplished with acetic anhydride is utilized to derivatize morphine to 3,6-diacetylmorphine for GC/MS analysis.The proposed method was precise and a wide linear range from 0.5 to 500 ngmL~(-1).A detection limit of 0.31 ng mL~(-1) in urine is achieved.The maximum ratio of morphine glucuronide to morphine in the blood of rat is 6 to 1.A terminal half-life of morphine in blood is calculated as around 120 min.