Reversible affinity interactions of antibody molecules at functionalized dendrimer monolayer:affinity-sensing surface with reusability

摘要

We described reversible affinity interactions of antibody molecules at a chemically functionalized electrode surface for a repeatedly renewable affinity-biosensing interface.Underlying biofunctionalizable monolayers were constructed with poly(amidoamine)dendrimers,whose surface chain-end groups were double-functionalized with biotinyl ligand and ferrocenyl groups for biospecific recognition and electron transfer reactions,respectively.Functionalized monolayers on gold electrodes provide platform surfaces for biospecific recognition reaction with monoclonal anti-biotin antibody molecules.Bound antibody molecules were dissociated from the surface via displacement reaction by the addition of free biotin in solutioin,enabling the affinity surface to be renewed and repeatedly untilized.Tracking of the association/dissociation reaction cycles were performed by registering the bioelectrocatalytic currents at the electrode using glucose oxidase(GO_x)as a signal generator and ferrocenyl-tethered dendrimer (F_c-D)as an electron transferring mediator in electrolyte.Shielding of the affinity surface by biospecifically bound antibody molecules caused hindrance in electron transfer,resulting in reduced signal from cyclic voltammetry.By the displacement reaction using free biotin,bound antibody molecules were dissociated from the surface and the bioelectrocatalytic signal was restored.With the affinity surfaces constructed in this work,continuous association/dissociation reactions have been successfully accomplised,providing a possibility of reusable affinity biosensing interface.