Study on the Plasma Protein Binding Rate and Compatibility Regularity of the Constituents Migrating to Blood of Simiao Yong’an Decoction | Bentham Science

摘要

Objective: To study the compatibility regularity of Simiao Yong’an decoction by determining the plasmaprotein binding rate with the constituents in Simiao Yong’an decoction and to preliminarily clarify the effects of thecompatibility on the plasma protein binding rate of different components.Methods: Based on the equilibrium dialysis method, high-performance liquid chromatography was used to determinethe contents of six constituents, which were divided into a single group and combination groups, in Simiao Yong’andecoction in the internal and external dialysis solutions. The obtained plasma protein binding rate through calculationswas an index to evaluate the binding of the above components to plasma protein in different conditions.Results: Harpagide, harpagoside, sweroside and loganin showed low plasma protein binding rates, ferulic acid exhibiteda moderate plasma protein binding rate, and glycyrrhizic acid showed a high plasma protein binding rate. Thecompatibility study showed that glycyrrhizic acid promoted the binding of ferulic acid to plasma protein. Glycyrrhizicacid and ferulic acid were the key compounds to promote the binding of harpagide to plasma protein. Glycyrrhizicacid, harpagide, harpagoside and loganin had a significant inhibitory effects on the binding of sweroside toplasma protein. The plasma protein binding capacities of harpagoside and loganin were reduced by the other fiveconstituents. Glycyrrhizic acid had the strongest plasma protein binding effect, and the binding effect was not affectedby other components.Conclusion: This study explores the effects of compound compatibility on effective components from the perspectiveof plasma protein binding by high-performance liquid chromatography combined with the equilibrium dialysismethod, and lays a foundation for clarifying the compatibility rule of Simiao Yong’an decoction and also provides anew idea for the study of the compatibility of traditional Chinese medicine formulas.