Preparation of a Novel NH_2~+ Ion Implantation Modified Electrode and Its Study of the Electrochemical Behavior of Hemoglobin

摘要

A novel functional electrode was obtained by implanting NH_2~+ into ITO film(NH2/ITO)for the first time.The NH2/ITO surface showed a better affinity to gold nanoparticles than bare ITO.Gold nanoparticles were deposited on the surface of NH2/ITO electrode(Au/NH2/ITO).The Au/NH2/ITO and NH2/ITO electrodes were used to observe the electrochemical behavior of Hemoglobin(Hb)immobilized on the electrodes surfaces.The peak current value of Hb immobilized on NH2/ITO increased compared with on bare ITO while peak current value of Hb immobilized on Au/NH2/ITO increased compared with on Au/ITO.Linkage between the-NH2 implanted into the ITO film and the-COOH of Hb was thought to be the reason for the increase of active Hb coverage on NH2/ITO compared with bare ITO.Increase of active Hb coverage on Au/NH2/ITO compare with Au/ITO was attributed to the different amount of gold nanoparticles deposited.Results showed the novel NH2/ITO and Au/NH2/ITO electrodes exhibited good stability,reproducibility besides selectivity and sensitivity.The electrode process of Hb immobilized on Au/NH2/ITO was quasi-reversible with adsorption.The electrode reaction rate constant k_s and other related constants were determined.X-ray photoelectron spectroscopy(XPS),field-emission scanning observation that up to 50% of this site marker, phenylbutazone, could be exchanged with TZH whilst only a few percent of ibuprofen were so affected.