Detection of PCB77 by Antibody-Coated Competitive Fluorescent Quantitative Immuno-PCR Using Molecular Beacon

摘要

A rapid and quantitative technique is urgently needed in detecting toxicological and carcinogenic polychlorinated biphenyls (PCBs) in the environment. In this study, a high sensitive real time fluorescent quantitative immuno-PCR (FQ-IPCR) approach using molecular beacon (MB) was developed to detect PCB77, which was classified as a human carcinogen. MB-based FQIPCR was then performed on serial dilutions of known PCB77 concentrations equivalent to 10-fold dilutions of 10–105 fg=mL. A correlation coefficient of 0.997 was identified, and a linear relationship between 10 fg=mL and 100 pg=mL, with y=0.543x+21.779, was obtained. Three soil samples were determined by MB-based FQ-IPCR to proof the validity of the method, and the results of which were further confirmed by conventional enzyme-linked immuno sorbent assay (ELISA). Based on sensitivity and reproduction, the MB-based real time FQ-IPCR technique will become a promising monitoring tool for environmental endocrine disruptors.