Electrochemical Investigation of Ascorbic Acid Interacting with Bovine Serum Albumin

摘要

Electrochemical investigation of the interaction of Ascorbic acid (AA) with bovine serum albumin (BSA) on a glassy carbon electrode is reported for the first time.In a 0.1 mol/l,pH 7.3 NaCl solution,AA had a well-defined voltammetric oxidative peak at +0.2086 V (versus Ag/AgCl) on a GC electrode.After the addition of BSA into the AA solution,the oxidative peak current decreased significantly without a shift of the peak potential,and no new peak appeared.The experimental results showed that a new nonelectrochemical supramolecular complex was formed after the interaction of BSA with AA,which resulted in a decrease of the diffusion coefficient,and then a decrease of the oxidative peak current.The interaction conditions and the electrochemical detection conditions were carefully investigated.The stoichi-ometry of this supramolecular complex was calculated based on voltammetric data with a binding number of two and a binding constant of 1.38 x 10~7.