Spectroscopic Probe-Aluminum(III)-Chrome Azure S Enhanced Determination of Serum Albumin in Microemulsion Medium

摘要

The sensitizing effect of spectroscopic probe and microemulsion media on the determination of serum albumin by spectrometry was developed.The main factors affecting the determination were investigated in detail.The results showed that serum albumin reacted with spectroscopic probe A1(III)-CAS to form a blue complex at pH = 3.0 in the p-octyl polyethyleneglycolphenyether microemulsion (Triton X-100-M).The lambda_(max) = 641 nm,and Beer's law was obeyed in the range of 0.0 and 35.0 mug centre dot ml-1 for bovine serum albumin (BSA).The sensitizing effect of spec- troscopic probe in Triton X-100-M microemulsion was higher than that in aqueous media.The proposed method has been used to determine protein in serum with satis- factory results.