Determination of Microcystins in Cyanobacteria by Supercritical Fluid Extraction and Liquid Chromatography-Tandem Mass Spectrometry

摘要

A method for the detection of microcystins (microcystin LR,RR,and YR) in cyanobacteria by supercritical fluid extraction (SFE) and liquid chromatography-mass spectrometry (LC/MS) has been developed.Supercritical fluids for the analytical extraction of nonvolatile,higher molecular weight compound,and microcystins from cyanobacteria were investigated.The microcystins included in this study are sparsely soluble in neat supercritical fluid CO2.However,the microcystins was successfully extracted with a ternary mixture (90% CO2,9.5% methanol,0.5% water) at 40°C and 250 atm.The polar carbon dioxide-aqueous methanol fluid system gave high extraction efficiency for the extraction of the polar microcystins from cyanobacteria.The microcystins were determined by liquid chromatography-tandem mass spectrometry (LC/MS/MS).