A photometric approach for detection of interaction of cells with their ligands based on lectin conjugated gold nanoparticles

摘要

A simple photometric approach for detection of interaction of lectins immobilized on colloidal gold particles with lymphocytes was demonstrated. It was shown that 20 nm gold particles conjugated with germ agglutinin or Concanavalin A undergo drastic optical changes upon interaction with lymphocytes. As a result of binding with cells, agglutination of gold-lectin-lymphocyte complex occurs with decrease of absorbance at 520 nm. This approach permitted to detect the interaction of 3000 lymphocytes/ml in 1.5h without any additional procedures.