MARKER-BASED SELECTION FOR THE YM4 BAMMV-RESISTANCE GENE IN BARLEY USING RAPDS

摘要

Studies to identify an RAPD marker linked to the BaMMV/BaYMV resistance gene ym4 were carried out on F-1 anther derived doubled haploid (DH) barley lines from a cross between the BaMMV/BaYMV susceptible cultivar 'Igri' and the resistant cultivar 'Franka' (ym4). For initial primer screening, bulked segregant analysis was used. Out of 148 decamer primers screened, only primer OP-Z04 revealed polymorphism resulting in an additional 660 bp band in the susceptible bulk. Linkage analysis carried out on 287 DH lines revealed that OP-Z04H660 is very closely linked to ym4 facilitating efficient marker-based selection for BaMMV/BaYMV resistance encoded by this gene. Additional studies showed that OP-Z04H660 can discriminate perfectly between resistant (ym4) and susceptible commercial barley cultivars.