Determination of Water Soluble Vitamins in Laboratory Animal Feeds by Micellar Electrokinetic Chromatography

摘要

Seven water-soluble vitamins: thiamine hydrochloride (B _1), riboflavin hydrochloride (B _2), pyridoxine hydrochloride (B _6), calcium pantotenate (B _5), ascorbic acid (C), folic acid (B _9), and biotin (H, B _7) were separated using the micellar electrokinetic chromatographic (MEKC) method in a single run with the electrolyte solution consisting of 100 mM H _3BO _3, 5 mM Na _2B _4O _7, and 30 mM sodium dodecylsulphate (SDS). The separation was achieved within 8 min. and the detection was performed at 200 nm. The calibration graphs plotted with six concentrations of each vitamin were linear with the determination coefficient r > 0.999. The method was applied for quantification of vitamins B _1, B _2, B _5, B _6, B _9, C, and H in novel feed for laboratory animals. The sample preparation involved solid-phase extraction of the vitamins, used prior to the MEKC analysis. The attained precision level was good with the recoveries between 98.4% and 105.6%. The simplicity of the procedure should make it highly useful for feed quality control in husbandry and the animal feed industry.